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Tuesday, July 28, 2020 | History

2 edition of Intracellular pH and the uptake of carcinostatic drugs by selected experimental tumors found in the catalog.

Intracellular pH and the uptake of carcinostatic drugs by selected experimental tumors

Richard Lee Hult

Intracellular pH and the uptake of carcinostatic drugs by selected experimental tumors

by Richard Lee Hult

  • 115 Want to read
  • 15 Currently reading

Published .
Written in English

    Subjects:
  • Antineoplastic agents.

  • Edition Notes

    Statementby Richard Lee Hult.
    The Physical Object
    Pagination132 leaves, bound :
    Number of Pages132
    ID Numbers
    Open LibraryOL14234395M

    We selected 4°C to investigate the energy impact on cellular uptake. With the reduced incubation temperature, the cellular uptake of PTX-CH Emul decreased dramatically in comparison to the control (37°C), suggesting that the endocytosis was an energy-dependent process (Figure 7A). When cells were preincubated with sucrose, cytochalasin D, or. Upcycling drugs for new or alternative purposes is beneficial and sustainable in general, as this reduces development costs, development time and the use of resources for development considerably. Examples include to-BBB’s formulation for treating brain tumors, 2B3–, and a formulation aimed at targeting neuroinflammation, 2B3–

      The group reported that the uptake of both compounds were pH dependent, where higher uptake at pH relative to that at pH was reported. It is interesting to note that an increase was only observed in V max with a decrease of pH from to and a negligible change was observed in K m at studied pH (Kobayashi et al. ). Intracellular brain pH and the pathway of a fat soluble pH indicator across the blood-brain barrier Brain Research, Vol. , No. 2 Factors that affect the uptake of ammonia by the brain: the blood-brain pH gradient.

      Similar efforts have been used to improve the pharmacological properties of LY and derivatives thereof. SF (compound 5, Figure 2) (Garlich et al., ) is a water soluble prodrug of. Drug carriers are also characterized by their ability to encapsulate drugs. This is commonly measured using two different parameters: drug-loading capacity (DLC) and drug-loading efficiency (DLE), as defined in Table a given amount of the drug carrier is unable to fully encapsulate a fixed quantity of the drugs, one would increase the amount of drug carrier to .


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Intracellular pH and the uptake of carcinostatic drugs by selected experimental tumors by Richard Lee Hult Download PDF EPUB FB2

Intracellular pH and the uptake of carcinostatic drugs by selected experimental tumors. Abstract. Graduation date: The relationship of intracellular drug levels and resultant drug activity to intracellular pH (pHi) was studied in the experimental tumors.

Glucose (5 g/kg) and/or sodium oxamate ( mmol) were administered to Walker INTRACELLULAR pH AND THE UPTAKE OF CARCINOSTATIC DRUGS BY SELECTED EXPERIMENTAL TUMORS I.

GENERAL INTRODUCTION In all things there is a poison, and there is nothing without poison. It depends only upon the dose whether a poison is poison or not That which redounds to the benefit of man is not poison; only that which is not of. In general, the extracellular pH (pHe) in human tumors is belowwhereas the intracellular pH (pHi) is maintained at neutral range, i.e., >, by powerful pHi control mechanisms.

The low pHe and the significant gradients between pHe and pHi affect markedly the response of tumors to various treatments such as chemotherapy, radiotherapy and. Further applications for cellular uptake experiments of established or investigational drugs include correlations between cytotoxicity and cellular drug content, investigations on the mechanisms of resistance which may involve decreased influx or increased efflux as compared to non-resistant cells, intracellular distribution of contrast agents Cited by: The intracellular pH (pH i) measured using the fluorescent ratio dye 2′,7′-bis(2-carboxyethyl)-5(6) carboxyfluorescein (BCECF) revealed expected differences between normal and cancer cells (low and high, respectively), and a mixed distribution in the MINO cells, with a subset of cells in the MINO having an increased rate of Cited by: 6.

1. Introduction. Intracellular pH (pH i) is known to be an important regulator of many cell normal cells, intracellular pH is lower than extracellular pH (pH e), with the pH i and pHe values lying mostly in the range – and – respectively.

Cancer cells are generally associated with higher values of pH i – and lower pH e – extracellular pH promotes experimental metastasis of human melanoma cells in athymic nude mice., Cancer Res. 66 () – doi / CAN The effect of pH on hyperthermic and x ray induced cell killing.

Int J Radiat Oncol Biol Phys. Feb; 7 (2)– [Google Scholar] Gillies RJ, Ogino T, Shulman RG, Ward DC. 31P nuclear magnetic resonance evidence for the regulation of intracellular pH by Ehrlich ascites tumor cells. J Cell Biol. Oct; 95 (1)–   The tumor cell line U87 was selected as the main cell model due to its overexpression of α v β 3 integrin receptors, and the MCF-7 cell line that has low expression of α v β 3 integrin receptors was used as a reference.

Compared with traditional small molecular drugs, siRNA requires more stringent conditions for drug carriers because of. Selenocompounds (SeCs) are well-known nutrients and promising candidates for cancer therapy; however, treatment efficacy is very heterogeneous and the mechanism of action is not fully understood.

Several SeCs have been reported to have albumin-binding ability, which is an important factor in determining the treatment efficacy of drugs.

In the present investigation, we. Oral NaHCO 3 therapy increases breast tumor pH in vivo from ± to ± and intracellular pH in breast epithelial organoids by ~ Breast tumors develop with median latency of ± days in NaHCO 3-treated mice vs. 82 ± days in control mice. This treatment regimen was shown to significantly increase the extracellular pH, but not the intracellular pH, of tumors by (31)P magnetic resonance spectroscopy and the.

Targeted drug delivery delivers the drug to the diseased tissue or cell while reducing the relative concentration of drugs in the remaining cells or tissues.

There are mainly two approaches for targeting: (1) passive targeting and (2) active targeting (Tekade et al., a, Tekade et al., b, Trafton, ).

Enhance absorption of the drugs into a selected tissue. Control the drug tissue distribution and pharmacokinetic.

Improve intracellular penetration. Prevent drugs from premature interaction with the biological environment. Reduce systemic toxicity. Particles with hydrodynamic diameters below 10 nm are subject to rapid kidney clearance. 1 day ago  Antibody-drug conjugates (ADCs) represent a novel and promising therapeutic strategy for the treatment of cancer patients.

ADCs target antigens highly expressed on the membrane surface of tumor cells to selectively deliver a cytotoxic drug. Ovarian tumors differentially express tumor-specific antigens, which can be used to guide ADCs. This strategy. Pancreatic cancer ranks among the tumors most resistant to chemotherapy.

Such chemoresistance of tumors can be mediated by various cellular mechanisms including dysregulated apoptosis or ineffective drug concentration at the intracellular target sites. In this review, we highlight recent advances in experimental chemotherapy underlining the role of cellular transporters in drug.

Reduced drug uptake is the third major mechanism responsible for β-lactam resistance in gram-negative bacteria, where β-lactams need to enter the periplasmic space to bind the PBP targets located in the cytoplasmic membrane.

In fact, in gram-negative bacteria, the activity of β-lactams against the bacterial cell depends on the complex interplay of a number of factors (Figure. Drug Targeting Principal schemes of drug targeting currently investigated in various experimental and clinical settings include: • Direct application of the drug into the affected zone (organ, tissue) • Passive accumulation of the drug through leaky vasculature (tumors, infarcts, inflammation) 2.

Drug delivery is the method or process of administering a pharmaceutical compound to achieve a therapeutic effect in humans or animals. For the treatment of. Abstract Quantitative assessment of the intracellular uptake of chlorotoxin in a U87 human glioma mouse model for the targeted drug delivery system Article Jul.

When cells are artificially exposed to basic pH values — for example, in cell culture experiments, fetal tissues (which typically have basic pH values of .Intracellular Se element was obviously increased upon treatment with p-XSC or ebselen for 30 min compared to basal levels (Figure 2 b), but this was not observed in the case of MeSeA or CysSe 2.

Co-treatment of 1% BSA with p-XSC or ebselen massively decreased the intracellular Se content down to approximately the basal level.A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text.